Anti-GSK3 beta Antibody (A80800)

Name: Anti-GSK3 beta Antibody
Brand: Antibodies.com
SKU: A80800

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This product is discontinued

Anti-GSK3 beta Antibody (A80800) has been discontinued and is no longer available.

View all Anti-GSK3 beta Antibodies.

Specifications

Name

Anti-GSK3 beta Antibody

Description

Rabbit polyclonal antibody to GSK3 beta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 321-420 of human GSK3ß (NP_001139628.1).

Sequence

LEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, GSK3B, GSK3beta isoform, GSK3B_HUMAN, Serine/threonine-protein kinase GSK3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GSK3 beta Antibody (A80800)

Western blot analysis of extracts of various cell lines, using Anti-GSK3 beta Antibody (A80800) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-GSK3 beta Antibody (A80800)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-GSK3 beta Antibody (A80800) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-GSK3 beta Antibody (A80800)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-GSK3 beta Antibody (A80800) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-GSK3 beta Antibody (A80800)

Immunofluorescence analysis of NIH-3T3 cells using Anti-GSK3 beta Antibody (A80800) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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