Anti-GSK3 beta Antibody [ARC0251] (A80603)

Name: Anti-GSK3 beta Antibody [ARC0251]
Brand: Antibodies.com
SKU: A80603

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This product is discontinued

Anti-GSK3 beta Antibody [ARC0251] (A80603) has been discontinued and is no longer available.

View all Anti-GSK3 beta Antibodies.

Specifications

Name

Anti-GSK3 beta Antibody [ARC0251]

Description

Rabbit monoclonal [ARC0251] antibody to GSK3 beta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human GSK3ß (P49841).

Sequence

PWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0251

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, GSK3B, GSK3beta isoform, GSK3B_HUMAN, Serine/threonine-protein kinase GSK3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GSK3 beta Antibody [ARC0251] (A80603)

Western blot analysis of extracts of various cell lines, using Anti-GSK3 beta Antibody [ARC0251] (A80603) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-GSK3 beta Antibody [ARC0251] (A80603)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-GSK3 beta Antibody [ARC0251] (A80603) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-GSK3 beta Antibody [ARC0251] (A80603)

Immunofluorescence analysis of HeLa cells using Anti-GSK3 beta Antibody [ARC0251] (A80603) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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