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Anti-GSK3 beta (phospho Ser21) Antibody [ARC54771] (A308976)

Western Blot - Anti-GSK3 beta (phospho Ser21) Antibody [ARC54771] (A308976) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC54771] antibody to GSK3 beta (phospho Ser21) for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-GSK3 beta (phospho Ser21) Antibody [ARC54771]
Description
Rabbit monoclonal [ARC54771] antibody to GSK3 beta (phospho Ser21).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S21&S9 of human GSK3a/GSK3ß.
Sequence
TSSFA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC54771
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
51 kDa / 49 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, GSK3B, GSK3beta isoform, GSK3B_HUMAN, Serine/threonine-protein kinase GSK3B
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-GSK3 beta (phospho Ser21) Antibody [ARC54771] (A308976) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-GSK3 beta (phospho Ser21) Antibody [ARC54771] (A308976)
Western blot analysis of extracts of NIH/3T3, using Anti-GSK3 beta (phospho Ser21) Antibody [ARC54771] (A308976) at 1:800 dilution. NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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