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Anti-PCNA Antibody (A34153)

Immunocytochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com Western blot - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com Immunoprecipitation - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com Immunohistochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.com
$390

Mouse monoclonal antibody to PCNA for WB, IP, ICC and IHC.

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Western Blot of PCNA in crude extract of S. cerevisiae. The antibody was used at 1:1,000 dilution. Molecular mass of PCNA is 29 kDa. Crude extract of S. cerevisiae strain BY4741 (20µg) applied.
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Anti-PCNA Antibody
Rabbit polyclonal antibody to PCNA for WB, IP and ChIP.
Name
Anti-PCNA Antibody
Description
Mouse monoclonal antibody to PCNA
Specificity
This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
Applications
WB, IP, ICC, IHC
Dilutions
Western blotting: 1:1000
Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant human PCNA protein fragments expressed in E.coli.
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Conjugate

Unconjugated

Formulation
ascites
Storage
store at -20˚C
Synonyms
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, PCNA_HUMAN, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunocytochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image
Figure 1: Immunocytochemistry - Anti-PCNA Antibody (A34153)
Immunocytochemistry staining of HeLa cells using anti-PCNA antibody (dilution 1: 100).Fixed in 100% methanol for 2hr at -20℃.
Western blot - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image
Figure 2: Anti-PCNA Antibody (A34153)
Western blot detection of PCNA antibody in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA antibody (1: 1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.
Immunoprecipitation - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image
Figure 3: Immunoprecipitation - Anti-PCNA Antibody (A34153)
Immunoprecipitation analysis of Hela cell lysates using PCNA antibody.
Immunohistochemistry - PCNA Monoclonal Antibody from Signalway Antibody (27210) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-PCNA Antibody (A34153)
Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA Mouse mAb (2E1-G10-H10,1: 400 diluted),showing nuclear localization. A high pressure mediated antigen retrieval step was performed in citrate buffer (pH6.0).
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