Anti-NeuN Antibody (A12719)

Name: Anti-NeuN Antibody
Brand: Antibodies.com
SKU: A12719
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NeuN for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Neuronal Marker

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Specifications

Name

Anti-NeuN Antibody

Description

Rabbit polyclonal antibody to NeuN.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of NeuN (NP_001076044.1).

Sequence

MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

46 - 55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLJ56884, FLJ58356, Fox-1 homolog C, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3, HRNBP3, neuronal nuclei, Rbfox3, RFOX3_HUMAN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox 1 homolog (C. elegans) 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-NeuN Antibody (A12719)

Western blot analysis of extracts of various cell lines, using Anti-NeuN Antibody (A12719) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-NeuN Antibody (A12719)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NeuN Antibody (A12719) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-NeuN Antibody (A12719)

Immunofluorescence analysis of rat brain cells using Anti-NeuN Antibody (A12719) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-NeuN Antibody (A12719)

Immunofluorescence analysis of mouse brain cells using Anti-NeuN Antibody (A12719) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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