Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200)

Name: Anti-STAT3 (phospho Ser727) Antibody [ARC0150]
Brand: Antibodies.com
SKU: A308200
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0150] antibody to STAT3 (phospho Ser727) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-STAT3 (phospho Ser727) Antibody [ARC0150]

Description

Rabbit monoclonal [ARC0150] antibody to STAT3 (phospho Ser727).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S727 of human Phospho-Phospho-STAT3-S727 (S727) (P40763).

Sequence

PMSPR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0150

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

88 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200)

Western blot analysis of extracts of various cell lines, using Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200) at 1:1,000 dilution. NIH/3T3 and PC-12 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.

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Figure 2: Immunohistochemistry - Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT3 (phospho Ser727) Antibody [ARC0150] (A308200) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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