Anti-STAT3 (phospho Ser727) Antibody (A91363)

Name: Anti-STAT3 (phospho Ser727) Antibody
Brand: Antibodies.com
SKU: A91363
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to STAT3 (phospho Ser727) for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-STAT3 (phospho Ser727) Antibody

Description

Rabbit polyclonal antibody to STAT3 (phospho Ser727).

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S727 of human Phospho-STAT3-S727 (NP_644805.1).

Sequence

PMSPR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Western blot analysis of extracts of HeLa cells, using Anti-STAT3 (phospho Ser727) Antibody (A91363) at 1:1,000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Western blot analysis of extracts of various cell lines, using Anti-STAT3 (phospho Ser727) Antibody (A91363) at 1:1,000 dilution. HepG2 cells and C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. HepG2 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-STAT3 (phospho Ser727) Antibody (A91363) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-STAT3 (phospho Ser727) Antibody (A91363)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-STAT3 (phospho Ser727) Antibody (A91363) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.