Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396)

Name: Anti-STAT3 (phospho Tyr705) Antibody [ARC50831]
Brand: Antibodies.com
SKU: A305396
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC50831] antibody to STAT3 (phospho Tyr705) for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-STAT3 (phospho Tyr705) Antibody [ARC50831]

Description

Rabbit monoclonal [ARC50831] antibody to STAT3 (phospho Tyr705).

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y705 of human Phospho-STAT3-Y705 (NP_644805.1).

Sequence

APYLK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50831

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

79 kDa / 88 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396)

Western blot analysis of NIH/3T3 cells, using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at 1:1,000 dilution. NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-STAT3 (phospho Tyr705) Antibody [ARC50831] (A305396) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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