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Anti-STAT3 (phospho Tyr705) Antibody (A16431)

Western Blot - Anti-STAT3 (phospho Tyr705) Antibody (A16431) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to STAT3 (phospho Tyr705) for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-STAT3 (phospho Tyr705) Antibody
Description
Rabbit polyclonal antibody to STAT3 (phospho Tyr705).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around Y705 of human Phospho-STAT3-Y705 (NP_644805.1).
Sequence
APYLK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
79 kDa / 86 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-STAT3 (phospho Tyr705) Antibody (A16431) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-STAT3 (phospho Tyr705) Antibody (A16431)
Western blot analysis of extracts of HeLa cells, using Anti-STAT3 (phospho Tyr705) Antibody (A16431) at 1:500 dilution. HeLa cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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