Anti-V5 Tag Antibody [ARC50435] (A306051)

Name: Anti-V5 Tag Antibody [ARC50435]
Brand: Antibodies.com
SKU: A306051
Price: 230.0 USD
Availability: InStock

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$230

Rabbit monoclonal [ARC50435] antibody to V5 Tag for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-V5 Tag Antibody [ARC50435]

Description

Rabbit monoclonal [ARC50435] antibody to V5 Tag.

Applications

WB, IP

Dilutions

WB: 1:2,000-1:20,000, IP: 1:2,000-1:6,000

Reactivity

Species Independent

Immunogen

A synthetic peptide corresponding to V5-Tag.

Sequence

GKPIPNPLLGLDST

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50435

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, rev, V5 epitope tag

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-V5 Tag Antibody [ARC50435] (A306051)

Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using Anti-V5 Tag Antibody [ARC50435] (A306051) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-V5 Tag Antibody [ARC50435] (A306051)

Western blot analysis of extracts of 293T cells, using Anti-V5 Tag Antibody [ARC50435] (A306051) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-V5 Tag Antibody [ARC50435] (A306051)

Immunoprecipitation analysis of 300µg extracts of CHO-V5 cells using 3µg of Anti-V5 Tag Antibody [ARC50435] (A306051). This Western blot was performed on the immunoprecipitate using Anti-V5 Tag Antibody [ARC50435] (A306051) at a dilution of 1:5000.

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