Recombinant Anti-Vimentin Antibody [rVIM/6431] (A250305)

Recombinant mouse monoclonal [rVIM/6431] antibody to Vimentin for WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

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Lead Time
4-6 Business Days
Telephone
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Highlighted products for Anti-Vimentin Antibody

Name
Anti-Vimentin Antibody [rVIM/6431]
Description
Recombinant mouse monoclonal [rVIM/6431] antibody to Vimentin.
Specificity
This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Rat, Horse, Chicken, Bovine, Feline, Canine, Porcine
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Recombinant fragment, within amino acids 2-466, of human Vimentin protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
rVIM/6431
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
57-60 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Vimentin Antibody [rVIM/6431] - BSA and Azide free (A253485).
Synonyms
CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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