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Anti-Vimentin (phospho Ser56) Antibody (A94802)

Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.com Immunohistochemistry - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.com Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to Vimentin (phospho Ser56) for WB, IHC and ELISA.

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Name
Anti-Vimentin (phospho Ser56) Antibody
Description
Rabbit polyclonal antibody to Vimentin (phospho Ser56).
Specificity
This antibody detects endogenous levels of Vimentin only when phosphorylated at Ser56.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:10000
Reactivity
Human
Immunogen
Synthetic peptide derived from human Vimentin around the phosphorylation site of Ser56 (amino acids 31-80).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
53kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A94802)
Western blot analysis of lysates from A549 cells treated with Nocodazole 1µg/ml 16h using Anti-Vimentin (phospho Ser56) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Vimentin (phospho Ser56) Antibody (A94802)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Vimentin (phospho Ser56) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A1243) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Vimentin (phospho Ser56) Antibody (A94802)
Western blot analysis of A549 cells using Anti-Vimentin (phospho Ser56) Antibody.
Publishing research using Anti-Vimentin (phospho Ser56) Antibody (A94802)? Please let us know so that we can list the citation on this page.

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