Anti-Calreticulin Antibody (A13022)

Name: Anti-Calreticulin Antibody
Brand: Antibodies.com
SKU: A13022
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Calreticulin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Cytoplasm Marker Cytoskeleton Marker ER Marker Nuclear Marker

Specifications

Name

Anti-Calreticulin Antibody

Description

Rabbit polyclonal antibody to Calreticulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-203 of human Calreticulineticulin (NP_004334.1).

Sequence

EPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Autoantigen RO, CALR, CALR protein, Calregulin, CALR_HUMAN, cC1qR, CRP55, CRT, CRTC, Endoplasmic reticulum resident protein 60, Epididymis secretory sperm binding protein Li 99n, ERp60, FLJ26680, grp60, HACBP, HEL S 99n, RO, Sicca syndrome antigen A, Sicca syndrome antigen A (autoantigen Ro; calreticulin), SSA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (10)

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Figure 1: Western Blot - Anti-Calreticulin Antibody (A13022)

Western blot analysis of extracts of various cell lines, using Anti-Calreticulin Antibody (A13022) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Calreticulin Antibody (A13022)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Calreticulin Antibody (A13022) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Calreticulin Antibody (A13022)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Calreticulin Antibody (A13022) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Calreticulin Antibody (A13022)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Calreticulin Antibody (A13022) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.