Recombinant Anti-Biotin Antibody [rBTN/8819] - BSA and Azide free (A315897)

Anti-Biotin Antibody [rBTN/8819] - BSA and Azide free

Recombinant mouse monoclonal [rBTN/8819] antibody to Biotin.

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Flow Cytometry, IF, WB, IHC-P

Biotinylated proteins in solution or on tissues.

Confocal Microscopy, In situ Nucleic Acid Hybridization, Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml, WB: 0.5-2 µg/ml, IHC: 0.5-1 µg/ml

Species Independent

Biotinylated sheep immunoglobulin.

Mouse

Monoclonal

rBTN/8819

kappa

Protein A/G chromatography.

1 mg/ml

244Da

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.