Anti-MARCKS Antibody [5F9] (A270556)

Immunofluorescent analysis of HeLa cell culture stained with Anti-MARCKS Antibody [5F9] (A270556), at a dilution of 1:1,000, in red, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:10,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Anti-MARCKS Antibody [5F9] (A270556) binds to MARCKS protein expressed in the membrane structures and cytoplasm, while Anti-Vimentin Antibody (A85421) stains the intermediate filaments.

Western blot analysis of different cell lysates using Anti-MARCKS Antibody [5F9] (A270556), in green. The lanes contain: [Lane 1] protein standard, [Lane 2] NIH-3T3 cells, [Lane 3] HEK293 cells, [Lane 4] HeLa cells, [Lane 5] SH-SY5Y cells, [Lane 6] C6 cells, and [Lane 7] COS1 cells. The band at ~80kDa corresponds to the MARCKS protein, detected only in the human and monkey cells. The antibody does not recognise the rodent form of the MARCKS protein.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human hippocampus section with Anti-MARCKS Antibody [5F9] (A270556) at a dilution of 1:2,000. Anti-MARCKS Antibody [5F9] (A270556) labels the nuclei, membrane and cytoplasm of glial cells and the synapses of neuronal cells. Note: this antibody performs well in the staining of NBF fixed human tissues but does not cross react with rodent.