Figure 1: Western Blot - Anti-CD34 Antibody [ARC55664] (A308717)
Western blot analysis of various lysates, using Anti-CD34 Antibody [ARC55664] (A308717) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:200000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD34 Antibody [ARC55664] (A308717) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD34 Antibody [ARC55664] (A308717) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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