Rabbit polyclonal antibody to MARCKS (phospho-Ser170)
Specificity
The antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from Human MARCKS.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
80kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
80K-L protein, MACS, Myristoylated alanine-rich C-kinase substrate, PKCSL, PRKCSL, Protein kinase C substrate, 80 kDa protein, light chain
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (Phospho-Ser170) Antibody #11535 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), using MARCKS (phospho-Ser170) Antibody #11535.
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