Recombinant rabbit monoclonal [RM251] antibody to AKT (phospho Ser473).
Spezifität
This antibody recognizes AKT only when phosphorylated at Ser473.
Anwendungen
WB, IHC
Verdünnungen
IHC: 1:200-1:500, WB: 1:1,000-1:2,000
Reaktivität
Human
Kreuzreaktivität
This antibody does not cross-react with AKT without phosphorylation at Ser473. This antibody may react with samples from bovine, mouse, and rat, as predicted by immunogen homology.
Immunogen
Synthetic peptide corresponding to human AKT (phospho Ser473).
Wirt
Rabbit
Klonalität
Monoclonal
Klon
RM251
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Protein A chromatography.
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1: Western Blot - Anti-AKT (phospho Ser473) Antibody [RM251] (A121248)
Western blot of lysates from 293 cells, either grown in medium with serum, serum starved, or insulin treated, using Anti-AKT (phospho Ser473) Antibody [RM251] at a 1:1,000 dilution or Anti-AKT1 Antibody [RM252] at a 1:1,000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-AKT (phospho Ser473) Antibody [RM251] at 1:400 dilution.
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