Anti-ATG4C Antikörper (A305074)

Name: Anti-ATG4C Antibody
Brand: Antibodies.com
SKU: A305074
Price: 500.0 USD
Availability: InStock

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$500

Polyklonal Kaninchen Antikörper gegen ATG4C für WB und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

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+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-ATG4C Antibody

Beschreibung

Rabbit polyclonal antibody to ATG4C.

Spezifität

Predicted molecular weight at ~52.5 kDa. Observed molecular weights at ~45-60 kDa.

Anwendungen

WB, ICC/IF

Verdünnungen

WB: 1:1,000, ICC/IF: 1:100

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide from the N-terminal of human ATG4C.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Peptide affinity purification.

Konzentration

1 mg/ml

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

APG4 autophagy 4 homolog C, APG4 autophagy 4 homolog C (S. cerevisiae), APG4 C, APG4-C, APG4C, ATG 4C, ATG4 autophagy related 4 homolog C, ATG4 autophagy related 4 homolog C (S. cerevisiae), ATG4C_HUMAN, AUT (S. cerevisiae) like 1, cysteine endopeptidase, AUT like 1, cysteine endopeptidase, AUT like 1, cysteine endopeptidase (S. cerevisiae), AUT like 3 cysteine endopeptidase, AUT-like 3 cysteine endopeptidase, AUTL1, AUTL3, Autophagin 3, Autophagin-3, Autophagy related 4C cysteine peptidase, Autophagy related cysteine endopeptidase 3, Autophagy related protein 4 homolog C, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C, Cysteine protease ATG4C, EC 3.4.22, FLJ14867, OTTHUMP00000010715

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-ATG4C Antibody (A305074)

Western blot analysis of rat liver showing detection of ~52.5kDa ATG4C protein using Anti-ATG4C Antibody (A305074) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~52.5kDa.

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Figure 2: Western Blot - Anti-ATG4C Antibody (A305074)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Anti-ATG4C Antibody (A305074) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~52.5kDa.

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Figure 3: Immunofluorescence - Anti-ATG4C Antibody (A305074)

Immunocytochemistry/Immunofluorescence analysis of mouse myoblast cell line (C2C12), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG4C Antibody (A305074), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.