Anti-ATG7 Antikörper (A305058)

Name: Anti-ATG7 Antibody
Brand: Antibodies.com
SKU: A305058
Price: 500.0 USD
Availability: InStock

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$500

Polyklonal Kaninchen Antikörper gegen ATG7 für WB, ICC/IF und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Peroxisome Marker

Produktdetails

Produktname

Anti-ATG7 Antibody

Beschreibung

Rabbit polyclonal antibody to ATG7.

Anwendungen

WB, ICC/IF, IHC

Verdünnungen

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide from the N-terminal of Human ATG7.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Peptide affinity purification.

Konzentration

1 mg/ml

Molekulargewicht

77.9 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

1810013K23Rik, Apg 7, Apg7, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7 like, APG7, S. cerevisiae, homolog of, APG7-like, APG7L, ATG 7, ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), Atg7l, ATG7_HUMAN, Autophagy 7, S. cerevisiae, homolog of, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, GSA7, hAGP7, Ubiquitin activating enzyme E1 like protein, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Immunohistochemistry - Anti-ATG7 Antibody (A305058)

Immunohistochemistry analysis of human brain, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-ATG7 Antibody (A305058) at 1:50 for 31 min at room temperature. Counterstain: Hematoxylin. Magnification: 10X.

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Figure 2: Western Blot - Anti-ATG7 Antibody (A305058)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~77.9 kDa ATG7 protein using Anti-ATG7 Antibody (A305058) at 1:1,000 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human Cervical cancer cell line (HeLa) lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~77.9 kDa.

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Figure 3: Western Blot - Anti-ATG7 Antibody (A305058)

Western blot analysis of rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Anti-ATG7 Antibody (A305058) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~77.9 kDa.

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Figure 4: Immunofluorescence - Anti-ATG7 Antibody (A305058)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG7 Antibody (A305058), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.