Anti-GAPDH Antikörper (A85377)

Name: Anti-GAPDH Antibody
Brand: Antibodies.com
SKU: A85377
Price: 185.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Novus (NB300-327). Entdecken Sie mehr für weniger.

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$185

Polyklonal Kaninchen Antikörper gegen GAPDH für WB, ICC/IF und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 4-6 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Cytoplasm Marker Cytoskeleton Marker Loading Control Nuclear Marker

Produktdetails

Produktname

Anti-GAPDH Antibody

Beschreibung

Rabbit polyclonal antibody to GAPDH.

Anwendungen

WB, ICC/IF, IHC

Verdünnungen

WB: 1:30,000, ICC/IF: 1:2,000, IHC: 1:2,000

Reaktivität

Human, Horse, Bovine, Porcine, Chicken, Rat, Mouse

Immunogen

Recombinant full-length human GAPDH, expressed in and purified from E. coli.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Molekulargewicht

36 kDa

Reinheit

Whole antiserum.

Produktform

Liquid

Formulierung

Supplied as an aliquot of serum with 5mM Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Immunofluorescence - Anti-GAPDH Antibody (A85377)

HeLa cells stained with Anti-GAPDH Antibody (red), Anti-TAF15 Antibody (A85450 | green) and DNA (blue). The Anti-GAPDH Antibody reveals strong cytoplasmic staining, while the Anti-TAF15 Antibody reveals a granular nuclear localization.

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Figure 2: Immunofluorescence - Anti-GAPDH Antibody (A85377)

Immunofluorescent analysis of HeLa cells stained with Anti-GAPDH Antibody (A85377), at a dilution of 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. The Anti-GAPDH Antibody (A85377) produces diffuse cytoplasmic staining of cells.

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Figure 3: Western Blot - Anti-GAPDH Antibody (A85377)

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using Anti-GAPDH Antibody (A85377), at a dilution of 1:20,000, in red. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with Anti-SF3B4 Antibody (A85417), at a dilution of 1:1,000, in green. In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.