Anti-GAPDH Antikörper [ARC50888] (A309068)

Anti-GAPDH Antikörper [1D4]

A85382

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Monoklonal [1D4] Maus Antikörper gegen GAPDH für WB, ICC/IF und IHC.

Anti-GAPDH Antikörper [GA1R]

A85271

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Monoklonal [GA1R] Maus Antikörper gegen GAPDH für Dot, ELISA, IS und WB.

(5)

A83722

Polyklonal Ziege Antikörper gegen GAPDH für ELISA, WB, IF und IHC.

(4)

A85377

Polyklonal Kaninchen Antikörper gegen GAPDH für WB, ICC/IF und IHC.

Anti-GAPDH Antikörper [1D4]

A85382

229 Produktreferenzen

Monoklonal [1D4] Maus Antikörper gegen GAPDH für WB, ICC/IF und IHC.

Anti-GAPDH Antikörper [GA1R]

A85271

18 Produktreferenzen

Monoklonal [GA1R] Maus Antikörper gegen GAPDH für Dot, ELISA, IS und WB.

(5)

A83722

Polyklonal Ziege Antikörper gegen GAPDH für ELISA, WB, IF und IHC.

(4)

A85377

Polyklonal Kaninchen Antikörper gegen GAPDH für WB, ICC/IF und IHC.

Produktdetails

Produktname

Anti-GAPDH Antibody [ARC50888]

Beschreibung

Rabbit monoclonal [ARC50888] antibody to GAPDH.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:10,000-1:1:120,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_002037.2).

Sequenz

VKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC50888

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

36 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (12)

Figure 1: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of extracts of HeLa cells, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 2: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of various lysates, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 3: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 4: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 5: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 6: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 7: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 8: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 9: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 10: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of NIH/3T3 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Figure 11: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of PC-12 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).