Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GSK3 beta Antibody (A305082), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Figure 2: Western Blot - Anti-GSK3 beta Antibody (A305082)
Western blot analysis of human Cell line lysates showing detection of GSK3 Beta protein using Anti-GSK3 beta Antibody (A305082) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GSK3 beta Antibody (A305082), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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