Anti-IL-2 Antikörper (A8383)

Western blot analysis of extracts of Jurkat cells, using Anti-IL-2 Antibody (A8383) at 1:1,000 dilution. Jurkat cells were treated by TPA (40 nM), A23187 (2µM) and Brefeldin A (300 ng / ml) for 0-24hoursThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Western blot analysis of extracts of Recombinant Human IL2 Protein, using Anti-IL-2 Antibody (A8383) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.