Anti-MLH1 Antikörper [AMC0417] (A309408)

Name: Anti-MLH1 Antibody [AMC0417]
Brand: Antibodies.com
SKU: A309408

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Dieses Produkt wurde eingestellt.

Anti-MLH1 Antikörper [AMC0417] (A309408) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-MLH1 Antikörper anzeigen.

Produktdetails

Produktname

Anti-MLH1 Antibody [AMC0417]

Beschreibung

Mouse monoclonal [AMC0417] antibody to MLH1.

Anwendungen

WB, IHC

Verdünnungen

WB: 1:100-1:500, IHC: 1:50-1:200

Reaktivität

Human

Immunogen

Recombinant protein of human MLH1.

Wirt

Mouse

Klonalität

Monoclonal

Klon

AMC0417

Isotyp

IgG1

Leichte Ketten

kappa

Konjugat

Unconjugated

Reinigung

Affinity purification.

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC, HNPCC 2, HNPCC2, MGC5172, MLH 1, MLH1_HUMAN, MutL homolog 1, MutL homolog 1 (E. coli), MutL homolog 1 colon cancer nonpolyposis type 2, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1, MutL, E. coli, homolog of, 1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (7)

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Figure 1: Western Blot - Anti-MLH1 Antibody [AMC0417] (A309408)

Western blot analysis of extracts of Jurkat cells, using Anti-MLH1 Antibody [AMC0417] (A309408) at 1:500 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-MLH1 Antibody [AMC0417] (A309408)

Western blot analysis of extracts from normal (control) and MLH1 Mouse mAb knockout (KO) HeLa cells, using Anti-MLH1 Antibody [AMC0417] (A309408) at 1:500 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-MLH1 Antibody [AMC0417] (A309408)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MLH1 Antibody [AMC0417] (A309408) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MLH1 Antibody [AMC0417] (A309408)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MLH1 expression (negative control samples) using Anti-MLH1 Antibody [AMC0417] (A309408) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.