Anti-Smad4 Antikörper [ARC5009-06] (A308139)

Name: Anti-Smad4 Antibody [ARC5009-06]
Brand: Antibodies.com
SKU: A308139
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC5009-06] Kaninchen Antikörper gegen Smad4 für WB, IHC, IP und ChIP.

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Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-Smad4 Antibody [ARC5009-06]

Beschreibung

Rabbit monoclonal [ARC5009-06] antibody to Smad4.

Anwendungen

WB, IHC, IP, ChIP

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).

Sequenz

PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC5009-06

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

65 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

(Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, DPC 4, DPC4, hSMAD4, JIP, MAD homolog 4, MAD mothers against decapentaplegic Drosophila homolog 4, MAD mothers against decapentaplegic homolog 4, MADH 4, MADH4, Med, Medea, Mothers against decapentaplegic homolog 4, Mothers against decapentaplegic, Drosophila, homolog of, 4, Mothers against DPP homolog 4, MYHRS, OTTHUMP00000163548, SMA- and MAD-related protein 4, SMAD 4, SMAD family member 4, SMAD mothers against DPP homolog 4, SMAD4_HUMAN

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (6)

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Figure 1: Anti-Smad4 Antibody [ARC5009-06] (A308139)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-Smad4 Antibody [ARC5009-06] (A308139), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB), as shown in figure.

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Figure 2: Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Western blot analysis of extracts of various cell lines, using Anti-Smad4 Antibody [ARC5009-06] (A308139) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

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Figure 4: Immunohistochemistry - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Smad4 Antibody [ARC5009-06] (A308139) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.