Anti-Fibrillarin Antibody (A89516)

Name: Anti-Fibrillarin Antibody
Brand: Antibodies.com
SKU: A89516
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Fibrillarin for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Nucleolar Marker Ribosomal Marker

Specifications

Name

Anti-Fibrillarin Antibody

Description

Rabbit polyclonal antibody to Fibrillarin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:20-1:50

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human Fibrillarin/U3 RNP (NP_001427.2).

Sequence

RGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

34 kD nucleolar scleroderma antigen, 34 kDa nucleolar scleroderma antigen, Fbl, FBRL_HUMAN, FIB, FIB1, FLRN, Histone-glutamine methyltransferase, Nop1p, RNA U3 small nucleolar interacting protein 1, RNU3IP1, rRNA 2' O methyltransferase fibrillarin, rRNA 2'-O-methyltransferase fibrillarin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Fibrillarin Antibody (A89516)

Western blot analysis of extracts of various cell lines, using Anti-Fibrillarin Antibody (A89516) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Immunofluorescence - Anti-Fibrillarin Antibody (A89516)

Immunofluorescence analysis of HeLa cells using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Fibrillarin Antibody (A89516)

Immunofluorescence analysis of C6 cells using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Fibrillarin Antibody (A89516)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).