Anti-AKT1 Anticorps (A308666)

Western blot analysis of extracts from wild type(WT) and AKT1 Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-AKT1 Antibody (A308666) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-AKT1 Antibody (A308666) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-AKT1 Antibody (A308666) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using Anti-AKT1 Antibody (A308666) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).