Anti-AKT1 (phospho Ser473) Anticorps [ARC0169] (A80471)

Name: Anti-AKT1 (phospho Ser473) Antibody [ARC0169]
Brand: Antibodies.com
SKU: A80471
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC0169] de lapin contre AKT1 (phospho Ser473) pour WB et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-AKT1 (phospho Ser473) Antibody [ARC0169]

Description du produit

Rabbit monoclonal [ARC0169] antibody to AKT1 (phospho Ser473).

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S473 of human Akt1 (P31749).

Séquence

QFSYS

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0169

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

60 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

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Figure 1: Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Western blot analysis of extracts of various cell lines, using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at 1:1,000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.