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Anti-Bcl-2 Anticorps (A93039)

Produits recommandés pour Anti-Bcl-2 Anticorps

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A86278
16 Références
Anti-Bcl-2 Anticorps [Bcl-2/100]
Anticorps monoclonal [Bcl-2/100] de souris contre Bcl-2 pour Flow Cytometry, IP, WB, IHC-P, IHC-Fr et ICC.
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A86278
16 Références
Anti-Bcl-2 Anticorps [Bcl-2/100]
Anticorps monoclonal [Bcl-2/100] de souris contre Bcl-2 pour Flow Cytometry, IP, WB, IHC-P, IHC-Fr et ICC.
Nom du produit
Anti-Bcl-2 Antibody
Description du produit
Rabbit polyclonal antibody to Bcl-2.
Applications
WB, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human Bcl-2 (P10415).
Séquence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
26 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Bcl-2 Antibody (A93039) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Bcl-2 Antibody (A93039)
Western blot analysis of extracts of Mouse lung, using Anti-Bcl-2 Antibody (A93039) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.
Western Blot - Anti-Bcl-2 Antibody (A93039) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Bcl-2 Antibody (A93039)
Western blot analysis of extracts of various cell lines, using Anti-Bcl-2 Antibody (A93039) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-Bcl-2 Antibody (A93039) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Bcl-2 Antibody (A93039)
Immunofluorescence analysis of NIH/3T3 cells using Anti-Bcl-2 Antibody (A93039) at a dilution of 1:250. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Bcl-2 Antibody (A93039) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-Bcl-2 Antibody (A93039)
Immunofluorescence analysis of HeLa cells using Anti-Bcl-2 Antibody (A93039) at a dilution of 1:250. DAPI was used to stain the cell nuclei (blue).

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