Anti-beta Tubulin Anticorps [AMC0498] (A80389)

Name: Anti-beta Tubulin Antibody [AMC0498]
Brand: Antibodies.com
SKU: A80389

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Ce produit est discontinué

Anti-beta Tubulin Anticorps [AMC0498] (A80389) a été abandonné et n'est plus disponible.

Voir toutes Anti-beta Tubulin Anticorps.

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Spécifications

Nom du produit

Anti-beta Tubulin Antibody [AMC0498]

Description du produit

Mouse monoclonal [AMC0498] antibody to beta Tubulin.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:5,000-1:10,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50- 1:200

Reactivité

Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii

Immunogène

Recombinant protein of human ß-Tubulin.

Hôte

Mouse

Clonalité

Monoclonal

Clone

AMC0498

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

50 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 1% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

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Figure 1: Western Blot - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Western blot analysis of extracts of various cell lines, using Anti-beta Tubulin Antibody [AMC0498] (A80389) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-beta Tubulin Antibody [AMC0498] (A80389)Immunohistochemistry analysis of paraffin-embedded rat ovary using beta-Tubulin Mouse mAb (AC021) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-beta Tubulin Antibody [AMC0498] (A80389)Immunohistochemistry analysis of paraffin-embedded rat stomach using beta-Tubulin Mouse mAb (AC021) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Immunofluorescence analysis of C6 cells using Anti-beta Tubulin Antibody [AMC0498] (A80389) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).