Anti-GAPDH Anticorps [ARC50888] (A309068)

Anti-GAPDH Anticorps [1D4]

A85382

229 Références

Anticorps monoclonal [1D4] de souris contre GAPDH pour WB, ICC/IF et IHC.

Anti-GAPDH Anticorps [GA1R]

A85271

18 Références

Anticorps monoclonal [GA1R] de souris contre GAPDH pour Dot, ELISA, IS et WB.

(5)

A83722

Anticorps polyclonal de chèvre contre GAPDH pour ELISA, WB, IF et IHC.

(4)

A85377

Anticorps polyclonal de lapin contre GAPDH pour WB, ICC/IF et IHC.

Anti-GAPDH Anticorps [1D4]

A85382

229 Références

Anticorps monoclonal [1D4] de souris contre GAPDH pour WB, ICC/IF et IHC.

Anti-GAPDH Anticorps [GA1R]

A85271

18 Références

Anticorps monoclonal [GA1R] de souris contre GAPDH pour Dot, ELISA, IS et WB.

(5)

A83722

Anticorps polyclonal de chèvre contre GAPDH pour ELISA, WB, IF et IHC.

(4)

A85377

Anticorps polyclonal de lapin contre GAPDH pour WB, ICC/IF et IHC.

Spécifications

Nom du produit

Anti-GAPDH Antibody [ARC50888]

Description du produit

Rabbit monoclonal [ARC50888] antibody to GAPDH.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:10,000-1:1:120,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_002037.2).

Séquence

VKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC50888

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

36 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (12)

Figure 1: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of extracts of HeLa cells, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 2: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of various lysates, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 3: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 4: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 5: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 6: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 7: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 8: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 9: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 10: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of NIH/3T3 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Figure 11: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of PC-12 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).