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Anti-His Tag Anticorps [ARC2696] (A309752)

Western Blot - Anti-His Tag Antibody [ARC2696] (A309752) - Antibodies.com Immunofluorescence - Anti-His Tag Antibody [ARC2696] (A309752) - Antibodies.com Western Blot - Anti-His Tag Antibody [ARC2696] (A309752) - Antibodies.com
Ce produit est discontinué

Anti-His Tag Anticorps [ARC2696] (A309752) a été abandonné et n'est plus disponible.

Voir toutes Anti-His Tag Anticorps.

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Anti-His Tag Anticorps [HIS.H8]
Anticorps monoclonal [HISH8] de souris contre HHHHHH tag pour Dot, ELISA, IP, IS et WB.
Nom du produit
Anti-His Tag Antibody [ARC2696]
Description du produit
Rabbit monoclonal [ARC2696] antibody to His Tag.
Applications
WB, ICC/IF, IP
Recommander des dilutions
WB: 1:2,000-1:6,000, ICC/IF: 1:50-1:200, IP: 1:10,000-1:30,000
Reactivité
Species Independent
Immunogène
A synthetic peptide corresponding to His tag.
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC2696
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
16 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-His Tag Antibody [ARC2696] (A309752) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-His Tag Antibody [ARC2696] (A309752)
Western blot analysis of extracts of His Protein, using Anti-His Tag Antibody [ARC2696] (A309752) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-His Tag Antibody [ARC2696] (A309752) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunofluorescence - Anti-His Tag Antibody [ARC2696] (A309752)
Immunofluorescence analysis of 293T-SOST His(C) and 293T using Anti-His Tag Antibody [ARC2696] (A309752) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-His Tag Antibody [ARC2696] (A309752) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Western Blot - Anti-His Tag Antibody [ARC2696] (A309752)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-His Tag Antibody [ARC2696] (A309752). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody [ARC2696] (A309752) at a dilution of 1:30000.

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