Anti-MDC1 Anticorps [P2B11] (A304945)

Name: Anti-MDC1 Antibody [P2B11]
Brand: Antibodies.com
SKU: A304945
Price: 465.0 USD
Availability: InStock

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$465

Anticorps monoclonal [P2B11] de souris contre MDC1 pour WB et ICC/IF.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-MDC1 Antibody [P2B11]

Description du produit

Mouse monoclonal [P2B11] antibody to MDC1.

Spécificité

This antibody recognizes MDC1 at and around the N-terminus.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:2,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Bovine, Chimpanzee

Immunogène

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Hôte

Mouse

Clonalité

Monoclonal

Clone

P2B11

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~184 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Homologue to Drosophila photoreceptor protein calphotin, MDC 1, MDC1_HUMAN, Mediation of DNA damage checkpoint 1, Mediator of DNA damage checkpoint 1, Mediator of DNA damage checkpoint protein 1, NFBD 1, NFBD1, Nuclear factor with BRCT domains 1, Nuclear Factor with BRCT Domains Protein 1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP: IgG at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 2: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: mouse Cortex. Lane 3: mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 3: Immunofluorescence - Anti-MDC1 Antibody [P2B11] (A304945)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-MDC1 Antibody [P2B11] (A304945), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.