Anti-p53 Anticorps (A309435)

Name: Anti-p53 Antibody
Brand: Antibodies.com
SKU: A309435
Price: 470.0 USD
Availability: InStock

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$470

Anticorps polyclonal de lapin contre p53 pour WB, IHC, ICC/IF, IP et ChIP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout ER Marker Mitochondrial Marker

Spécifications

Nom du produit

Anti-p53 Antibody

Description du produit

Rabbit polyclonal antibody to p53.

Applications

WB, IHC, ICC/IF, IP, ChIP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000, ChIP: 1:1,000-1:5,000

Reactivité

Human

Immunogène

Recombinant protein of human p53.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

53 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Western Blot - Anti-p53 Antibody (A309435)

Western blot analysis of extracts of 293T cells, using Anti-p53 Antibody (A309435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-p53 Antibody (A309435)

Western blot analysis of extracts from wild type (WT) and p53 knockout (KO) 293T cells, using Anti-p53 Antibody (A309435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-p53 Antibody (A309435)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p53 Antibody (A309435) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-p53 Antibody (A309435)

Confocal immunofluorescence analysis of Hela cells using Anti-p53 Antibody (A309435) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).