Anti-p53 (phospho Ser33) Anticorps (A90465)

Name: Anti-p53 (phospho Ser33) Antibody
Brand: Antibodies.com
SKU: A90465
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre p53 (phospho Ser33) pour WB, IHC et IP.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Mots clés :

ER Marker Mitochondrial Marker

Spécifications

Nom du produit

Anti-p53 (phospho Ser33) Antibody

Description du produit

Rabbit polyclonal antibody to p53 (phospho Ser33).

Applications

WB, IHC, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, IP: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S33 of human p53 (NP_000537.3).

Séquence

VLSPL

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

55 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-p53 (phospho Ser33) Antibody (A90465)

Western blot analysis of extracts of HT-29 cells, using Anti-p53 (phospho Ser33) Antibody (A90465) at 1:2,000 dilution or p53 antibody (A3185). HT-29 cells were treated by Nocodazole (100ng/ml) for 16 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-p53 (phospho Ser33) Antibody (A90465)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-p53 (phospho Ser33) Antibody (A90465) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-p53 (phospho Ser33) Antibody (A90465)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-p53 (phospho Ser33) Antibody (A90465) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-p53 (phospho Ser33) Antibody (A90465)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-p53 (phospho Ser33) Antibody (A90465) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Anti-p53 (phospho Ser33) Anticorps (A90465)

Anticorps polyclonal de lapin contre p53 (phospho Ser33) pour WB, IHC et IP.

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Livraison

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Produits alternatifs à Anti-p53 (phospho Ser33) Anticorps (A90465)