Anti-ROCK1 Anticorps (A12783)

Name: Anti-ROCK1 Antibody
Brand: Antibodies.com
SKU: A12783
Price: 470.0 USD
Availability: InStock

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$470

Anticorps polyclonal de lapin contre ROCK1 pour WB et IHC.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-ROCK1 Antibody

Description du produit

Rabbit polyclonal antibody to ROCK1.

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Séquence

YSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

158 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

coiled-coil-containing protein kinase 1, coiled-coil-containing protein kinase I, MGC131603, MGC43611, p160 Rhoassociated coiled coil-forming protein kinase, p160 ROCK-1, p160 ROCK1, p160ROCK, PRO0435, Renal carcinoma antigen NY REN 35, Renal carcinoma antigen NY-REN-35, Rho associated coiled coil containing protein kinase 1, Rho associated protein kinase 1, Rho kinase, Rho-alpha kinase, Rho-associated, Rho-associated protein kinase 1, ROCK I, ROCK-I, ROCK1_HUMAN, Rok, rokalpha

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of various lysates, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of C6, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-ROCK1 Antibody (A12783)

Western blot analysis of extracts from wild type(WT) and ROCK1 knockout (KO) 293T cells, using Anti-ROCK1 Antibody (A12783) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-ROCK1 Antibody (A12783)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-ROCK1 Antibody (A12783) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.