Anti-SMAD5 (phospho Ser463 + Ser465) Anticorps (A308813)

Name: Anti-SMAD5 (phospho Ser463 + Ser465) Antibody
Brand: Antibodies.com
SKU: A308813
Price: 435.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$435

Anticorps polyclonal de lapin contre SMAD5 (phospho Ser463 + Ser465) pour WB et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-SMAD5 (phospho Ser463 + Ser465) Antibody

Description du produit

Rabbit polyclonal antibody to SMAD5 (phospho Ser463 + Ser465).

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S463 & S465 of human Phospho-Smad5-S463/S465 (NP_005894.3).

Séquence

SSVS

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Western blot analysis of extracts of NIH/3T3, using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at 1:600 dilution. NIH/3T3 cells were treated by BMP4 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SMAD5 (phospho Ser463 + Ser465) Antibody (A308813) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.