Anti-V5 Tag Anticorps [ARC50445] (A306050)

Name: Anti-V5 Tag Antibody [ARC50445]
Brand: Antibodies.com
SKU: A306050

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Ce produit est discontinué

Anti-V5 Tag Anticorps [ARC50445] (A306050) a été abandonné et n'est plus disponible.

Voir toutes Anti-V5 Tag Anticorps.

Spécifications

Nom du produit

Anti-V5 Tag Antibody [ARC50445]

Description du produit

Rabbit monoclonal [ARC50445] antibody to V5 Tag.

Applications

WB, ICC/IF, IP

Recommander des dilutions

WB: 1:2,000-1:6,000, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000

Reactivité

Species Independent

Immunogène

A synthetic peptide corresponding to V5-Tag.

Séquence

GKPIPNPLLGLDST

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC50445

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

40 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, rev, V5 epitope tag

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-V5 Tag Antibody [ARC50445] (A306050)

Western blot analysis of extracts of CHO cells, using Anti-V5 Tag Antibody [ARC50445] (A306050) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-V5 Tag Antibody [ARC50445] (A306050)

Immunofluorescence analysis of 293T cells transfected with V5(mCherry) protein and untreated 293T cells using Anti-V5 Tag Antibody [ARC50445] (A306050) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Western Blot - Anti-V5 Tag Antibody [ARC50445] (A306050)

Immunoprecipitation analysis of 300µg extracts of CHO-V5 cells using 30µg of Anti-V5 Tag Antibody [ARC50445] (A306050). This Western blot was performed on the immunoprecipitate using Anti-V5 Tag Antibody [ARC50445] (A306050) at a dilution of 1:3000.