Anti-Vinculin Anticorps [ARC51900] (A305534)

Name: Anti-Vinculin Antibody [ARC51900]
Brand: Antibodies.com
SKU: A305534
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC51900] de lapin contre Vinculin pour WB, ICC/IF et IP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

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Spécifications

Nom du produit

Anti-Vinculin Antibody [ARC51900]

Description du produit

Rabbit monoclonal [ARC51900] antibody to Vinculin.

Applications

WB, ICC/IF, IP

Recommander des dilutions

WB: 1:10,000-1:30,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).

Séquence

DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC51900

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

124 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, VINC_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)

Western blot analysis of various lysates, using Anti-Vinculin Antibody [ARC51900] (A305534) at 1:250,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-Vinculin Antibody [ARC51900] (A305534)

Confocal imaging of HeLa cells using Anti-Vinculin Antibody [ARC51900] (A305534), at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 3: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Vinculin Antibody [ARC51900] (A305534). This Western blot was performed on the immunoprecipitate using Anti-Vinculin Antibody [ARC51900] (A305534) at a dilution of 1:1000.