This antibody recognises human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane. Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.
Applications
WB, IHC-P, IP, Flow Cytometry, IHC-Fr, IF
Recommander des dilutions
WB: 1:500 - 1:2,000, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivité
Human, Rat, Rabbit, Chicken, Mouse
Immunogène
Purified vinculin from human platelets.
Hôte
Mouse
Clonalité
Monoclonal
Clone
V284
Isotype
IgG1
Conjuguer
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Stockage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Notes générales
Mouse anti Human vinculin antibody, clone V284 recognizes human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane.Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.