Anti-His Tag Antibody (A306183)

Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Anti-His Tag Antibody (A306183) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using Anti-His Tag Antibody (A306183) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation analysis of 300µg extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag using 3µg of Anti-His Tag Antibody (A306183). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody (A306183) at 1:5,000 dilution.

Immunoprecipitation analysis of extracts of cells using Anti-His Tag Antibody (A306183). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody (A306183) at a dilition of 1:3000.