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Recombinant Anti-His Tag Antibody [RM146] (A121323)

Western Blot - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.com Immunoprecipitation - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.com Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.com Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121223) - Antibodies.com
Recombinant Antibody
$510

Recombinant rabbit monoclonal [RM146] antibody to His Tag for WB, IP, ICC, Flow Cytometry and IHC.

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Anti-His Tag Antibody [AMC0149]
Mouse monoclonal [AMC0149] antibody to His Tag for WB and IP.
Name
Anti-His Tag Antibody [RM146]
Description
Recombinant rabbit monoclonal [RM146] antibody to His Tag.
Specificity
This antibody recognizes recombinant proteins containing the 6 x His Tag or 10 x His Tag, fused to either the amino or carboxy terminus.
Applications
WB, IP, ICC, Flow Cytometry, IHC
Dilutions
WB: 0.1-1 µg/ml, IP: 0.5-2 µg/ml, ICC: 0.5-2 µg/ml, FC: 0.5-2 µg/ml,IHC: 0.1-1 µg/ml
Reactivity
All Species
Cross Reactivity
There is no cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogen
Mixture containing peptide with His Tag (N terminal) and peptide with His Tag (C terminal).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM146
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-His Tag Antibody [RM146] (A121323)
Western blot of 293T cells, (1) untransfected or (2) transfected with fusion protein X (His tag), and E. coli lysate, (3) without Protein Y (His tag) or (4) with Protein Y (His tag), using Anti-His Tag Antibody [RM146] at 0.2µg/ml.
Immunoprecipitation - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.comEnlarge Image
Figure 2: Immunoprecipitation - Anti-His Tag Antibody [RM146] (A121323)
Immunoprecipitation of 293T cells expressing a His Tag fusion protein by Anti-His Tag Antibody [RM146], and then blotted with Anti-His Tag Antibody [RM146]. (1) Whole lysate control. (2) IP by rabbit IgG control. (3) IP by Anti-His Tag Antibody [RM146].
Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323)
Immunohistochemistry staining of naive HepG2 cells (Negative control) using Anti-His Tag Antibody [RM146].
Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121223) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323)
Immunohistochemistry staining of 293T cells expressing nuclear protein X (His tag), using Anti-His Tag Antibody [RM146].
Publishing research using Recombinant Anti-His Tag Antibody [RM146] (A121323)? Please let us know so that we can list the citation on this page.

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