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Anti-HSPA1A Antibody (A11133)

Western Blot - Anti-HSPA1A Antibody (A0284) - Antibodies.com Western Blot - Anti-HSPA1A Antibody (A0284) - Antibodies.com Immunofluorescence - Anti-HSPA1A Antibody (A0284) - Antibodies.com Immunofluorescence - Anti-HSPA1A Antibody (A0284) - Antibodies.com
This product is discontinued

Anti-HSPA1A Antibody (A11133) has been discontinued and is no longer available.

View all HSPA1A Antibodies.

  • Specifications

Name
Anti-HSPA1A Antibody
Description
Rabbit polyclonal antibody to HSPA1A.
Applications
WB, IF, IP
Dilutions
WB: 1:500-1:2000, IF: 1:50-1:200, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide of human HSPA1A.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
72kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, Heat shock protein family A member 1A, Heat shock protein family A member 1B, HSP70-1, HSP70-2, HSP70.1, HSP70.2, HSP72, HSPA1, HSPA1B, HSX70
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-HSPA1A Antibody (A0284) - Antibodies.comEnlarge Image

Western Blot - Anti-HSPA1A Antibody (A11133)

Western blot analysis of extracts of various cell lines, using Anti-HSPA1A Antibody (A0284) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.
Western Blot - Anti-HSPA1A Antibody (A0284) - Antibodies.comEnlarge Image

Western Blot - Anti-HSPA1A Antibody (A11133)

Western blot analysis of extracts of various cells, using Anti-HSPA1A Antibody (A0284) at 1:1,000 dilution ratio through one-step method. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:4000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence - Anti-HSPA1A Antibody (A0284) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-HSPA1A Antibody (A11133)

Immunofluorescence analysis of U2OS cells using Anti-HSPA1A Antibody (A0284). Blue: DAPI for nuclear staining.
Immunofluorescence - Anti-HSPA1A Antibody (A0284) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-HSPA1A Antibody (A11133)

Immunofluorescence analysis of NIH/3T3 cells using Anti-HSPA1A Antibody (A0284) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Proteins predicted to interact with HSP70

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
98.9% Relevancy Score
98.5% Relevancy Score
98% Relevancy Score
97.7% Relevancy Score
96.9% Relevancy Score
95.6% Relevancy Score
95.6% Relevancy Score
94.1% Relevancy Score
93.9% Relevancy Score