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Anti-HSP70 Antibody (A305017)

Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A305017) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A305017) - Antibodies.com Western Blot - Anti-HSP70 Antibody (A305017) - Antibodies.com
$520
Product Datasheet

Chicken polyclonal antibody to HSP70 for WB, ICC/IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-HSP70 Antibody
Description
Chicken polyclonal antibody to HSP70.
Applications
WB, ICC/IF, ELISA
Dilutions
WB: 1:1,000, ICC/IF: 1:120
Reactivity
Human, Mouse, Rat
Immunogen
Full length Human HSP70.
Host
Chicken
Clonality
Polyclonal
Isotype
IgY
Conjugate

Unconjugated

Purification
PEG purification.
Concentration
1 mg/ml
Molecular Weight
~70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, Heat shock protein family A member 1A, Heat shock protein family A member 1B, HSP70-1, HSP70-2, HSP70.1, HSP70.2, HSP72, HSPA1, HSPA1A, HSPA1B, HSX70
Isotype Controls
Suitable Secondaries
See all Anti-Chicken IgY Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A305017) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-HSP70 Antibody (A305017)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP70 Antibody (A305017), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Chicken (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody (A305017) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-HSP70 Antibody (A305017)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP70 Antibody (A305017), at 1:120 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Chicken (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Western Blot - Anti-HSP70 Antibody (A305017) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP70 Antibody (A305017)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of HSP70 protein using Anti-HSP70 Antibody (A305017) at 1:1,000 for 2 hours at room temperature. Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Goat anti-Chicken IgY: HRP for 1 hour at room temperature.
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Proteins predicted to interact with HSP70

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
98.9% Relevancy Score
98.5% Relevancy Score
98% Relevancy Score
97.7% Relevancy Score
96.9% Relevancy Score
95.6% Relevancy Score
95.6% Relevancy Score
94.1% Relevancy Score
93.9% Relevancy Score