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Anti-HSP70 Antibody [3A3] (A305077)

Western Blot - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.com Western Blot - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.com
$485
Product Datasheet

Mouse monoclonal [3A3] antibody to HSP70 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-HSP70 Antibody [3A3]
Description
Mouse monoclonal [3A3] antibody to HSP70.
Specificity
This antibody may detect HSP70, HSC70, p75, and HSP72.
Applications
WB, IHC, ICC/IF, IP, Antibody Microarray
Dilutions
WB: 1:5,000, ICC/IF: 1:500, IP: 2µg
Reactivity
Human, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Drosophila, Bacteria, Brine Shrimp
Immunogen
Recombinant human HSP70 protein, expressed in and purified from E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
3A3
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, Heat shock protein family A member 1A, Heat shock protein family A member 1B, HSP70-1, HSP70-2, HSP70.1, HSP70.2, HSP72, HSPA1, HSPA1A, HSPA1B, HSX70
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP70 Antibody [3A3] (A305077)

Western blot analysis of rat cell lysates showing detection of Hsp70 protein using Anti-HSP70 Antibody [3A3] (A305077) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Western Blot - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.comEnlarge Image

Western Blot - Anti-HSP70 Antibody [3A3] (A305077)

Western blot analysis of Artemia franciscanna (brine shrimp) cell lysates showing detection of Hsp70 protein using Anti-HSP70 Antibody [3A3] (A305077) at 1:1,000.
Immunocytochemistry/Immunofluorescence - Anti-HSP70 Antibody [3A3] (A305077) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-HSP70 Antibody [3A3] (A305077)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-HSP70 Antibody [3A3] (A305077), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Cytoplasm. Magnification: 40X.
Publishing research using Anti-HSP70 Antibody [3A3] (A305077)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with HSP70

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
98.9% Relevancy Score
98.5% Relevancy Score
98% Relevancy Score
97.7% Relevancy Score
96.9% Relevancy Score
95.6% Relevancy Score
95.6% Relevancy Score
94.1% Relevancy Score
93.9% Relevancy Score