Human, Mouse, Rat, Bovine, C. elegans, Beluga, Canine, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Monkey, Porcine, Plant, Rabbit, Sheep, Xenopus
Immunogen
Human Recombinant HSP70/HSC70.
Host
Mouse
Clonality
Monoclonal
Clone ID
N27F3-4
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~72 kDa (HSP) / ~73 kDa (HSC)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, Heat shock protein family A member 1A, Heat shock protein family A member 1B, HSP70-1, HSP70-2, HSP70.1, HSP70.2, HSP72, HSPA1, HSPA1A, HSPA1B, HSX70
Western Blot - Anti-HSP70 Antibody [N27F3-4] (A305087)
Western blot analysis of human Cell lysates showing detection of Hsp70 protein using Anti-HSP70 Antibody [N27F3-4] (A305087) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP70 Antibody [N27F3-4] (A305087) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.
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