Anti-MARCKS Antibody [5F9] (A270556)

Immunofluorescent analysis of HeLa cell culture stained using Anti-MARCKS Antibody [5F9] (A270556), at a dilution of 1:1,000, in red. The tissue was co-stained using Anti-Vimentin Antibody (A85421), at a dilution of 1:10,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Anti-MARCKS Antibody [5F9] (A270556) binds to MARCKS protein expressed in the membrane structures and in cytoplasm, while the Anti-Vimentin Antibody (A85421) stains the intermediate filaments.

Western blot analysis of different cell lysates using Anti-MARCKS Antibody [5F9] (A270556), in green. The lanes contain samples of: [1] Protein standards, [2] NIH-3T3 cells, [3] HEK293 cells, [4] HeLa cells, [5] SH-SY5Y cells, [6] C6 cells, and [7] COS1 cells. The band at about 80kDa corresponds to the MARCKS protein, detected only in the human and monkey cells. The antibody does not recognise the rodent form of the MARCKS protein.