Anti-MARCKS Antibody (A104335)

Name: Anti-MARCKS Antibody
Brand: Antibodies.com
SKU: A104335
Price: 185.0 USD
Availability: InStock

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$185

Chicken polyclonal antibody to MARCKS for WB, ICC/IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Specifications

Name

Anti-MARCKS Antibody

Description

Chicken polyclonal antibody to MARCKS.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:5,000-1:10,000, ICC/IF: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivity

Human, Monkey

Cross Reactivity

This antibody is not recommended for use on rodent material.

Immunogen

Recombinant full-length human MARCKS, expressed in and purified from E. coli.

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Conjugate

Unconjugated

Molecular Weight

~80 kDa (by SDS-PAGE)

Purity

IgY preparation.

Product Form

Liquid

Formulation

Supplied as an aliquot of IgY preparation with 5mM Sodium Azide.

Storage

Shipped at 4°C. For short-term storage, store at 4°C. For long-term storage, mix 1:1 with glycerol and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

80 kDa protein, 80K L, 80K L protein, 80K-L protein, 80KL, 81 kDa protein, light chain, light chain, MACS, MARCS, MARCS_HUMAN, MGC52672, myristoylated alanine rich C kinase substrate, Myristoylated alanine rich protein kinase C substrate, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L), Myristoylated alanine-rich C-kinase substrate, Phosphomyristin, PKCSL, PRKCSL, Protein kinase C substrate, protein kinase C substrate 80 kDa protein light chain

Isotype Controls

Chicken IgY (A63673)

Suitable Secondaries

See all Anti-Chicken IgY Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-MARCKS Antibody (A104335)

Western blot analysis of different cell lines lysates using Anti-MARCKS Antibody (1:1,000 | green): [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with Anti-GAPDH Antibody (1:5,000 | red), which reveals a single band at 37kDa in all preparations

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Figure 2: Immunofluorescence - Anti-MARCKS Antibody (A104335)

Immunofluorescent analysis of HeLa cells stained with Anti-MARCKS Antibody (1:5,000 ) red, and co-stained with Anti-beta Tubulin Antibody (1:10,000 | green). The blue is Hoechst staining of nuclear DNA. The Anti-MARCKS Antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the Anti-beta Tubulin Antibody stains cytoplasmic microtubules.

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