Anti-MARCKS Antibody (A85457)

Immunofluorescent analysis of HeLa cells stained with Anti-MARCKS Antibody (1:1,000 | red) and Anti-Beta Tubulin Antibody (A85428 | 1:10,000 | green). The blue is DAPI staining of nuclear DNA. Anti-MARCKS Antibody recognizes protein expressed in the plasma membrane and cytoplasm, while the Anti-Beta Tubulin Antibody stains microtubules.

View of mixed neuron/glial cultures stained with Anti-MARCKS Antibody (red) and Anti-MAP2 Antibody (A85363 | green). Note that the Anti-MARCKS Antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the Anti-MAP2 Antibody.

Western blot analysis of cell line lysates probed simultaneously with Anti-MARCKS Antibody (1:1,000 | green) and Anti-GAPDH Antibody (A85382 | 1:5,000 | red): [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6 cells. The strong band at 80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [5], but no reactivity is seen on rodent cells. Anti-GAPDH Antibody was used as a loading control - revealing a single band at ~37kDa in all preparations.

Western blot of whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with Anti-MARCKS Antibody (1:10,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~80 kDa corresponds to MARCKS.